PRAGUE MEDICAL REPORT
PRAGUE MEDICAL REPORT
Prague Medical Report is an English quarterly published multidisciplinary biomedical journal. Prague Medical Report was founded as Sborník lékařský in May 1885. The journal presents public primary scientific publications, short communications, casuistry, and reviews. It contains articles based on important specialised lectures and symposia.

PRAGUE MEDICAL REPORT, Vol 120 No 1 (2019), 5–9

Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug Absorption

Martin Šíma, Nikolina Kutinová-Canová, Pavel Ryšánek, Jana Hořínková, Denisa Moškořová, Ondřej Slanař

DOI: https://doi.org/10.14712/23362936.2019.5
published online: 20. 05. 2019

abstract

Data on gastric pH in rats to be used in preclinical models for pH-dependent drug absorption are still limited or contradictory. The aim of this study was to describe gastric pH in rats at fasted state and to evaluate its changes induced by pentagastrin or omeprazole in order to mimic gastric pH at fasted and fed human subjects. Twenty Wistar rats, fasting for 12 h, were randomly assigned into four treatment groups (n=5): control, pre-treated with omeprazole 2 h before pH measurement, pre-treated with omeprazole 12 h before pH measurement, and pre-treated with pentagastrin 20 min before pH measurement. An incision on the stomach wall was made in anesthetized animals, and pH of gastric juice was measured. The observed pH values were significantly different among groups (p=0.0341), with the median (IQR) values of gastric pH of 3.5 (2.7–4.2), 6.7 (4.7–7.0), 5.6 (3.5–6.4) and 2.2 (1.6–3.1) in control, omeprazole 2 h, omeprazole 12 h and pentagastin group, respectively. We recommend using short interval pentagastrin and 2 h omeprazole pre-treatment in fasting animals to model similar gastric pH as is expected in human fasted and fed state pharmacokinetic studies, respectively.

keywords: Gastric pH; Rats; Oral drug absorption; Omeprazole; Pentagastrin

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Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug Absorption is licensed under a Creative Commons Attribution 4.0 International License.

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ISSN: 1214-6994
E-ISSN: 2336-2936

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